Phytase Expressed by pIA 8 and pGAPZ A Vectors and Analysis of its Biochemical Characters
نویسنده
چکیده
Phytase and phytase gene from Aspergillus ficuum (A. ficuum) were used in this study. The results showed that phytase activity reached the peak of 0.17 U/g after 4 d incubation in solid medium for A. ficuum; the optimum pH and temperature of phytase were 2.5 and 50 o C, respectively. A 1.4-kb DNA containing the coding region of phytase gene was isolated and inserted into the expression vectors of pIA 8 and pGAPZ A, which were transformed into E. coli (Top 10). The maximal phytase activities in the supernatant and cells were 2.31 and 9.04 U/ml for the E. coli with pIA 8, 8.04 and 2.93 U/ml for the E. coli with pGAPZ A, respectively. It was concluded that the recombinant of pIA 8-phytase could express intracellular phytase, while the recombinant of pGAPZ A-phytase could express extracellular phytase. The molecular weight of phytase protein was 54.61 kDa.
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